Omics Research // Genomics
Targeted Gene Sequencing
Drill down on your human, mouse, rat, and other non-human targets' genetic sequence with amplicon and hybrid capture techniques.
What Is Targeted Sequencing?
Targeted sequencing is a methodology whereby distinct genomic regions of interest to the organism you’re studying are captured or selected for deeper sequencing depth.
Why Is Targeted Sequencing Important?
For scientists only interested in certain regions of the genome, targeted sequencing is more efficient and cost-effective than whole-genome sequencing or whole-exome sequencing and can yield specific, in-depth insights.
It can detect disease-related variants, inherited germline mutations, and CRISPR gene editing events. It can also target well-characterized variants within exons and methylation regions, as well as low-frequency variants.
What Can You Do?
Find Regions of High Interest
Pinpoint the exact region of nucleic acid that can efficiently unlock the answers you seek
Use Multiple Methodologies
A targeted approach and deeper sequencing depth make it easier to identify variants
Discover Disease Variants
Choose the best enrichment strategy — hybrid capture, amplicon, or custom — for your target region
Precision Medicine
Introduce new ways to prevent, diagnose, and treat disease on a personal level
Technologies Used for Targeted Sequencing
16S/ITS Sequencing
Best for
Identifying the taxonomy of the bacteria and fungi population within a sample by sequencing the hypervariable regions of the prokaryotic rRNA 16S gene and ITS gene
Benefits
A fast, affordable method to identify the taxonomic community of bacteria and fungi in environmental specimens
Custom Targeted Sequencing
Best for
Efficient enrichment for known, discovery-generated targets or hotspots when off-the-shelf options don’t apply.
Benefits
Easy-to-use custom design options from vendors such as IDT, Agilent, and Twist Bioscience enable both amplicon sequencing and hybrid capture-based sequencing
On-Going Promotions
We're always adding new deals — check in often for updated promotions!
Targeted Sequencing Best Practices
Sample Preparation & DNA Extraction
DNA extraction from blood, cultured cells, tissue, FFPE, saliva, swab, fecal, urine, or DNA for research use
Get your high-quality genomic DNA isolated from most sample types at Psomagen.
Sample Requirements
- 1ug of genomic DNA
- DNA concentration ≥ 20 ng/μl
- DIN ≥ 7.0 for non-FFPE, DIN ≥ 4.0 for FFPE
Library Preparation
- Agilent SureSelect custom panels
- Twist custom panel
- IDT xGen™ Custom Hyb Panels
- Illumina AmpliSeq
Instrumentation
Instrumentation
- Illumina NovaSeq 6000
- Illumina MiSeq
Bioinformatic Analysis
Basic analysis:
- Data quality control
- Mapping
- Variant analysis (SNP/InDels)
Advanced analysis:
- Structural variant (SV)
- Copy number variation (CNV)
- Annotation and statistics
- Family trio analysis
- Tumor-normal paired analysis
- Custom analysis
Why Our Clients Choose Psomagen
Superior Data Quality
Exceeds manufacturer benchmarks
Fast Turnaround
Starting at one week for sequencing projects
US-Based Processing
Sequencing and customer support in Rockville, MD
Ph.D. Level Support
Expert assistance every step of the way, with real-time project updates through our online system
Automated Workflows
Increase scalability and improve reproducibility
CLIA-Certified, CAP-Accredited Labs
Clinical research options available (inquire for more information)
Complete Sequencing Solutions
From extraction to data analysis, with many sample types accepted
Quotes From Our Clients
We chose you because we’ve used you before. Your services met our needs, you have good communication, and are fast responding.
Laboratory Administrator
University Research Laboratory
We chose Psomagen because of their CLIA/CAP accreditation, data quality, and price.
Postdoctoral Researcher
University Research Laboratory
Psomagen’s data quality, turnaround times, prices, and services met our needs.
Laboratory Manager
University Research Laboratory
We're Ready for Your Order!
Here's what to expect in your project's life cycle. If you have a question during sequencing, it's easy to get in touch!
01
Request a Consultation
Create an account through our client portal and get in touch with your local field team to start designing a project.
We’ll help drive your research forward with the right tools for your research needs.
02
Place an Order
After finalizing the scope of work, the official quote will be issued for signing. Then, your sales representative will send specific shipping instructions.
Once all the samples and necessary documents are ready, we can start your project. Need more analysis? Reach out to our bioinformatics experts to dig deeper.
03
QC and Data Delivery
All Psomagen sequencing projects come with a built-in QC step to ensure you get the information you need. An account manager will update you on your project's progress in a timely manner.
You can also see some project information and securely view all valid reports on the client portal site.
Targeted Sequencing in Action
More Sequencing Possibilities
Choose from our wide range of sequencing options — whole genome to single-cell transcriptomics — to support your project.