Omics Research // Metagenomics
16S/ITS Amplicon Sequencing
Identify the taxonomy of a sample's bacterial and fungal population by sequencing the hypervariable regions of the prokaryotic rRNA 16S gene and ITS gene
What Is 16S Sequencing?
Next-generation sequencing of the 16s rRNA gene —or just 16S sequencing, is a well-established and low-cost method to determine the microbial composition and diversity of bacteria in a sample. The presence of the 16S rRNA gene in almost all bacteria is very useful for analyses of the human, plant, animal, and environmental microbiome.
What Is ITS Sequencing?
Sequenced-based analysis of the Internal Transcribed Spacer (ITS) DNA sequence is regarded as the standard for taxonomic and diversity analyses of fungi. Given that this region is broadly present in fungi it is commonly used to identify species and perform genetic analyses of microbiomes, from human to environmental samples.
Why Are 16S and ITS Sequencing Important?
Both are inexpensive and effective methods to survey multiple samples at once, allowing fast and comprehensive characterization of the different bacterial and fungal populations within a sample.
What Can You Do?
Clinical Microbiology
Discover pathogenic microorganisms from clinical samples, including invasive bacterial and fungal infections
Microbial Ecology
Study the relationship microorganisms have with humans, plants, animals, and the environment
Freshwater / Wastewater Monitoring
Evaluate water quality, prevent operational problems at drinking water facilities (e.g. corrosion of iron pipes and modification of water properties by microorganisms), and monitor and prevent outbreaks
Food Science
Evaluate the contribution of microorganisms to the quality (e.g. flavor and texture) and safety (e.g. pathogen presence) of food
Applications of 16S and ITS Sequencing
16S rRNA Amplicon Sequencing
Amplify and sequence the V3-V4 region of the prokaryotic 16S rRNA gene to classify the phylum, class, order, family, genus, and species in a heterogeneous microorganism sample.
ITS Region Amplicon Sequencing
Classify the taxonomy of the fungi present in your diverse populations by amplifying and sequencing a ~210 bp region of the nuclear ribosomal internal transcribed spacer (ITS).
Custom Amplicon Sequencing
Do you have your own regions of interest to target using amplicon next-generation sequencing? Contact Psomagen for a consultation on your custom project needs!
On-Going Promotions
We're always adding new deals — check in often for updated promotions!
16S/ITS Sequencing Best Practices
Sample Preparation & DNA Extraction
DNA extraction from stool, swabs, saliva, urine, media, and more
Sample QC - >20 ng
Get your high-quality genomic DNA isolated from most sample types at Psomagen.
Library Preparation
16S rRNA Amplicon Library Prep (V3-V4 region)
- 12.5 ng genomic DNA
- Library QC >4 nM at ~630 bp
ITS rRNA Amplicon Library Prep (ITS2 region)
- 12.5 ng genomic DNA
- Library QC >4 nM at 275-725 bp
Sequencing Depth & Read Length
Instrumentation: Illumina MiSeq v3
- Read length: 2 x 300 bp
- >100,000 reads/sample
- 30% PhiX Spike-In
Psomagen can only guarantee raw sequencing depth
Bioinformatic Analysis
Basic analysis:
- Assembly and OTU clustering
- Taxonomic assignment
- Species abundance
- Diversity statistics
Data Delivery
All sequencing run data is delivered as demultiplexed .fastq files.
If an entire flow cell or lane is purchased, the raw data can be delivered as BCL files if requested at the time of the contract.
Psomagen can also provide taxonomic information on your bacterial or fungal samples using clustering in operational taxonomic unity (OTU) analysis.
Why Our Clients Choose Psomagen
Superior Data Quality
Exceeds manufacturer benchmarks
Fast Turnaround
Starting at one week for sequencing projects
US-Based Processing
Sequencing and customer support in Rockville, MD
Ph.D. Level Support
Expert assistance every step of the way, with real-time project updates through our online system
Automated Workflows
Increase scalability and improve reproducibility
CLIA-Certified, CAP-Accredited Labs
Clinical research options available (inquire for more information)
Complete Sequencing Solutions
From extraction to data analysis, with many sample types accepted
Quotes From Our Clients
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
We're Ready for Your Order!
Here's what to expect in your project's life cycle. If you have a question during sequencing, it's easy to get in touch!
01
Request a Consultation
Create an account through our client portal and get in touch with your local field team to start designing a project.
We’ll help drive your research forward with the right tools for your research needs.
02
Place an Order
After finalizing the scope of work, the official quote will be issued for signing. Then, your sales representative will send specific shipping instructions.
Once all the samples and necessary documents are ready, we can start your project. Need more analysis? Reach out to our bioinformatics experts to dig deeper.
03
QC and Data Delivery
All Psomagen sequencing projects come with a built-in QC step to ensure you get the information you need. An account manager will update you on your project's progress in a timely manner.
You can also see some project information and securely view all valid reports on the client portal site.
16S/ITS Sequencing in Action
More Sequencing Possibilities
Choose from our wide range of sequencing options — whole genome to single-cell transcriptomics — to support your project.