Omics Research // Genomics
Bacterial Genome Sequencing
Understand pathogen genetics and identify targets for treatment with bacterial genome sequencing using long-read sequencing technology.
What Is Bacterial Genome Sequencing?
Bacterial genome sequencing (BGS) is a sequencing method used to characterize bacterial pathogens. This methodology provides insights into virulence, antibiotic resistance, and other properties important to researchers.
Why Is Bacterial Genome Sequencing Important?
Bacterial diseases are a major cause of illness and morbidity in humans and animals. Increased incidence of antibiotic resistance makes it important to identify mutations and develop additional treatments.
Long reads are a valuable tool for bacterial genome sequencing. R10.4.1 flow cells can generate near-complete bacterial genomes. This eliminates the need for short-read assembly, which can struggle to resolve highly repetitive regions.
What Can You Do?
Classify Bacteria
Find evolutionary links between strains of bacteria. Classify unknown microbes.
Monitor Outbreaks
Identify bacteria and mutations responsible for infectious disease outbreaks.
Explore Microbial Diversity
Understand bacterial diversity in an environment.
Personalized Medicine
Characterize host-pathogen interactions and develop appropriate treatments.
Bacterial Genome Sequencing Best Practices
Sample Requirements
- Sample type: single species bacterial DNA
- Concentration: 100–150 ng/µl
- Purity: A260/280 = 1.8–2.0
- Volume: 15 µl/sample
- Size: Up to 12M bases
Sample Size
Small (1~6Mb)
- Concentration: 100–150 ng/µl
- Volume: 15 µl
- Coverage: 100x
- Price: $90/sample
Large (6~12Mb)
- Concentration: 100-150 ng/µl
- Volume: 15 µl
- Coverage: 100x
- Price: $110/sample
Accuracy
v14 library prep chemistry paired with R10.4.1 flow cells, raw data accuracy is >99%
Quality Check
Targeting data size based on the targeting coverage. Any results that do not meet the targeting data size can be translated to insufficient gDNA.
Sequencing results are directly dependent on the quantity, quality, and purity of sample DNA.
Results
File types:
- FASTA (consensus sequence of the genome)
- GenBank (.gbk file)
- FASTQ (all the raw sequencing reads)
Turnaround Time
Small sample (<6Mb): 1–2 business days
Large sample (<12Mb): 3–4 business days
gDNA extraction service: additional 2–3 business days (coming soon)
Troubleshooting
Causes of inadequate sample quality:
- Insufficient DNA concentration
- Verification on Qubit
- Degraded and/or contaminated samples
- Poor gDNA extraction technique
- Reagents remaining in final product
- Multiple freeze/thaw cycles
- Exposure to extreme temperatures
- Multi-species sample
- This service is intended for single-species bacterial samples. Non-bacterial or multi-species samples may fail.
Why Our Clients Choose Psomagen
Superior Data Quality
Exceeds manufacturer benchmarks
Fast Turnaround
Starting at one week for sequencing projects
US-Based Processing
Sequencing and customer support in Rockville, MD
Ph.D. Level Support
Expert assistance every step of the way, with real-time project updates through our online system
Automated Workflows
Increase scalability and improve reproducibility
CLIA-Certified, CAP-Accredited Labs
Exceeds manufacturer benchmarks
Complete Sequencing Solutions
From extraction to data analysis, with many sample types accepted
The Right Method for You
Find a match for your next sequencing project with one of Psomagen's tried-and-true services.