Omics Research // Transcriptomics
mRNA Sequencing
Use the poly-A tail of mRNA transcripts, positively selecting mRNA out of all the background RNA, to isolate the coding RNA sequences of actively transcribed genes. Obtain functional analysis of transcriptional regulators in disease and baseline biological processes.
What Is mRNA Sequencing?
mRNA sequencing is the method by which coding RNA in the form of messenger RNA (mRNA) is selected by the poly-A tail and sequenced using next-generation sequencing.
Why Is mRNA Sequencing Important?
RNA transcription is the link between DNA and protein expression. Using mRNA-seq enables the user to analyze gene expression patterns by sequencing less raw throughput while maintaining high mappable sequencing coverage.
Studying the RNA present gives scientists insight into the biology of a cell and how changes — alternative splicing events, gene fusions, and allele-specific expression of rare and novel messages — may relate to disease.
What Can You Do?
Positive Selection
mRNA selection using the poly-A tail of extracted RNA
Strand-Specific Sequencing
Identify which strand of DNA the transcript was derived from
High Specificity
Positive selection leads to uniform coverage of RNA that codes for proteins and other polyadenylated non-coding RNA
Experienced Bioinformatics Experts
Basic gene expression and SNV/InDel or advanced bioinformatic analysis services
Technologies Used for mRNA Sequencing
Stranded mRNA Seq
Psomagen uses strand-specific mRNA sequencing, or stranded mRNAseq, to identify overlapping transcripts and genes.
Researchers can use the strand orientation information in their analyses to aid in annotation and novel transcript calls.
Gene Expression Profiling
Gene expression profiling analyzes known genes. Transcripts are assembled with StringTie software based on a reference genome model.
After assembly, the abundance of gene/transcript is calculated in the read count and normalized value as FPKM (Fragments Per Kilobase of transcript per Million mapped reads) for each sample. Isoforms of known transcripts are provided.
On-Going Promotions
We're always adding new deals — check in often for updated promotions!
mRNA Sequencing Best Practices
Sample Preparation & RNA Extraction
Total RNA extraction from cells, tissue, FFPE, blood, and swab samples
- Sample QC: >500 ng and RIN >7.0
- FFPE: >200 ng and DV 200 >70%
For blood samples, globin mRNA depletion can be performed to reduce its abundance and investigate other mRNAs.
Have a low amount of samples? Take a look at our low-input RNA seq to find the right option for you.
Library Preparation
- Stranded mRNA
- 500 ng Total RNA (25-1000 ng)
- Library QC >10 nM at ~260 bp
Sequencing Instrumentation & Read Length
Illumina NovaSeq 6000
- 2 x 150 bp read length
- Custom read depth
Bioinformatic Analysis
Basic analysis:
- Mapping
- Gene expression profiling
- Variant analysis (SNV, InDels)
Advanced analysis:
- Novel alternative transcripts
- Fusion gene list
- Differentially Expressed Gene (DEG)
- KEGG Enrichment Analysis
- Alternatively spliced transcripts
Data Delivery
All sequencing run data is delivered as demultiplexed .fastq files
If the entire flowcell or lane is purchased, the raw data can be delivered as BCL files if requested at the time of contract.
Why Our Clients Choose Psomagen
Superior Data Quality
Exceeds manufacturer benchmarks
Fast Turnaround
Starting at one week for sequencing projects
US-Based Processing
Sequencing and customer support in Rockville, MD
Ph.D. Level Support
Expert assistance every step of the way, with real-time project updates through our online system
Automated Workflows
Increase scalability and improve reproducibility
CLIA-Certified, CAP-Accredited Labs
Clinical research options available (inquire for more information)
Complete Sequencing Solutions
From extraction to data analysis, with many sample types accepted
Quotes From Our Clients
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
We're Ready for Your Order!
Here's what to expect in your project's life cycle. If you have a question during sequencing, it's easy to get in touch!
01
Request a Consultation
Create an account through our client portal and get in touch with your local field team to start designing a project.
We’ll help drive your research forward with the right tools for your research needs.
02
Place an Order
After finalizing the scope of work, the official quote will be issued for signing. Then, your sales representative will send specific shipping instructions.
Once all the samples and necessary documents are ready, we can start your project. Need more analysis? Reach out to our bioinformatics experts to dig deeper.
03
QC and Data Delivery
All Psomagen sequencing projects come with a built-in QC step to ensure you get the information you need. An account manager will update you on your project's progress in a timely manner.
You can also see some project information and securely view all valid reports on the client portal site.