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NGS Order Guide

Quotation is available upon project consultation

Sample Requirement for Genome Service

DNA Quality

DNA Sample  should be double-strand, non degraded, and containing no particulate matter (A260/280 ≥ 1.7). DIN > 7.0 is required, except for special instances. The amount requirement depends on the kit to be used. Please consult with your representative.

RNA Quality

RNA samples should have RIN > 7.0. The amount requirement depends on the kit to be used. Please consult with your representative.

Sample Preparation Guide

Genomic DNA RequirementsRNA RequirementsVenous Blood RequirementsSaliva RequirementsPre-made Library
Submit minimum concentration of 20 ng/µl Submit minimum concentration of 20 ng/µlGenomic DNARecommend Oragene·DISCOVER (DNA Genotek) for collection and storageLibrary must be compatible with Illumina platform
500 ng for whole genome sequencing500ng for bulk RNA sequencingCollect minimum of 2 mL of venous blood in EDTA coated vacutainer (BD)Follow manufacturer’s instruction closely for collectionSubmit minimum of 10µl at 5nM for QC and Sequencing
1 µg for whole exome sequencingWe use 3-5µl of sample for QCStore collected blood in freezer or refrigerator prior to shippingStore collected sample at Room TemperatureqPCR quantification (KAPA biosystems)
We use 3-5µl of sample for QCRNA must be intact and free of genomic DNARNAD5000 or D1000 ScreenTape by Agilent TapeStation (Agilent Technologies)
DNA must be intact and high molecular weightRecommend TE bufferCollect minimum of 8 mL of venous blood in PAXgene Blood RNA Tubes (Qiagen)
Samples must not be PCR-amplifiedIncubate collected blood at Room Temperature for at least 2 hours prior to freezing or refrigeration
Recommend TE (10 mM Tris/1 mM EDTA) buffer

Sample QC Method

Genomic DNA RNAShipping Requirements
gDNA ScreenTape by Agilent TapeStation RNA ScreenTape by Agilent TapeStation Label all sample with legible unique Sample ID
DNA integrity Number (DIN) used to assess integrityRNA integrity Number (RIN) used to assess integrityPlace sample in screw-cap or snap top tube sealed with parafilm
Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher Scientific) used to accurately quantify double strand DNAQuant-iT RicoGreen RNA Assay Kit (ThermoFisher Scientific) used to accurately quantify RNAUse leak-proof container, Ziploc bag or larger screw-cap tube, with enough absorbent materials
Blood and Saliva samples must be sent according to local, state, federal, IATA, and ICAO regulations with appropriate biological substance tag
Send samples in box with sufficient dry ice to keep sample frozen during shipping except Saliva should be sent at Room Temperature

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