Next-Generation Sequencing Service

genetic linkage with blue and red lights surrounded

What Is Next-Generation Sequencing?

With sequencing methods of the past, such as the Sanger method, it could take as long as a decade to decode the human genome. Today, next-generation sequencing (NGS) technology has made it possible to do the same job in just a few days. NGS applications enable unprecedented scalability and touch on all areas of DNA research, including whole-genome sequencing and exome sequencing. These sensitive and contemporary sequencing methods enable researchers to discover a greater volume of submicroscopic genetic mutations in our DNA, and they’re paving the way for future genetic breakthroughs.

How Does Psomagen NGS Technology Work?

There are three main steps to the NGS workflow, including library preparation, sequencing, and bioinformatics. First, the DNA strand is fragmented into smaller strands. Adapter-ligated fragments are amplified, or copied, using the PCR (polymerase chain reaction) method to produce a large number of DNA clusters.

From here, NGS platforms can sequence millions of small fragments of DNA in parallel, which means that massive amounts of DNA data can be generated more quickly and cost-effectively than ever before. Researchers can choose a wide view to look at multiple samples or zoom in for an in-depth analysis of a small, specific area of interest.

Applications of Next-Generation Sequencing

NGS enables researchers to uncover valuable genetic information that expands future discoveries. The latest in NGS technology can be applied to many applications, ranging from short to long reads, DNA and RNA, and epigenome and metagenome applications. Explore more applications below.

Whole Genome

Achieve maximum efficiency without losing quality while analyzing the complete set of genes.

Exome & Targeted

Investigate focused genomic regions to identify variations in the protein-coding part of a selected gene.

RNA/Transcriptome

Enable rapid profiling and deep investigation of the transcriptome in a given cell.

Epigenome

Analyze the chemical modifications to the DNA that may shut off or turn on a gene.

Metagenome

Microbial sequencing customized to your research for efficient identification of microorganism diversity.

Low Pass Sequencing

Provides an accurate and cost-effective solution to measuring genome-wide genetic variation.

Bioinformatics

Combines computer science, statistics, mathematics and engineering to study and process biological data.

Health Care Applications of Next-Generation Sequencing

NGS is the future of DNA research and health care; the applications of this technology have the potential to enhance individualized patient care. Costs are coming down, and the rate at which we are able to sequence DNA is rapidly increasing, which makes cutting-edge technology accessible to more researchers.

NGS allows DNA specialists to gather large amounts of genetic data, which has huge applications to health care. This sophisticated sequencing method is helping us better understand how the expression of genetic variants affects us. For example, NGS allows scientists to more easily identify exome mutations, which are thought to contain the vast majority of mutations that lead to human diseases. With NGS, we can efficiently compare the DNA of thousands of people and explore the individual genes that cause conditions such as cancer, schizophrenia, and autism.

More NGS health applications include:

Identification of rare genetic mutations and variations

Identification of rare genetic mutations and variations

A deeper analysis of targeted DNA regions

A deeper analysis of targeted DNA regions

Advanced analysis of gene expression

Advanced analysis of gene expression

Discovery of cancer-causing genetic mutations

Discovery of cancer-causing genetic mutations

More rapid whole-genome sequencing

More rapid whole-genome sequencing

Microbiology research into bacteria and viruses

Microbiology research into bacteria and viruses

Reproductive and genetic health

Reproductive and genetic health

Next-Generation Sequencing vs. Sanger Sequencing

The methods of NGS and Sanger sequencing are similar, but they differ in terms of the amount of DNA you can work through at one time. While the traditional Sanger method only captures substitutions and small insertions and deletions, NGS captures a wider range of substitutions, inversions, translocations insertions, and deletions of DNA. It allows clinicians to sequence up to thousands of genes at the same time. This makes NGS a more cost-effective solution than Sanger sequencing for a high number of targets.

Deep NGS is also much more sensitive than Sanger sequencing, which allows us to discover brand-new mutations and disease-causing genes and enables science to find rare genetic variants. More specifically, NGS technology is helping researchers define the genomes of pathogens that may contain details about drug sensitivities and the cause of outbreaks. It is completely changing the way researchers approach problems and is uncovering exciting, unprecedented solutions.

What to Expect When You Work With Psomagen

Taking advantage of NGS requires some extra computer capacity and the expertise to understand all the new data acquired. Clinics may not have the resources, but we do. When you make us your next-generation sequencing service provider, you can expect the latest solutions from our modern, fully equipped lab, and you’ll have full support from our clinicians during every phase of your research, whether it’s your first or your hundredth DNA project.

For over 15 years, Psomagen Inc. has provided a wide variety of genetic technology services to more than 100 countries, and we’re here to support you in the steps of NGS, including:

Sample extraction v2

Sample extraction

Library preparation

Library preparation

Amplification

Amplification

Sequencing v2

Sequencing

About Psomagen, Inc.

As a CLIA-licensed research facility, we offer highly specialized sequencing services and utilize the latest next-gen sequencing. Psomagen employs highly trained researchers who are experts in their fields. Our next-generation sequencing services have been utilized by researchers from many prestigious institutions, including the National Institute of Health (NIH), the Food and Drug Administration (FDA), Stanford University, and many more.

Macrogen, Inc., the parent company, was founded in 1997, and in 2005, Macrogen Corp. was established in the U.S. In 2012, Macrogen Corp. was established to provide advanced clinical sequencing services to U.S. patients and hospitals. During this time, we’ve become a leading bioscience research firm and our reputation in the industry is proof of our commitment to advanced human genomic sequencing. In 2019, the name was changed so Psomagen, Inc. to better represent the vision of the company.

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