Research

Whole Exome Sequencing Services

Psomagen Inc. offers a way for variant discovery using whole exome sequencing services with Next-Generation sequencing platforms on a research level for most eukaryotic species that has coding region known as exon.

Psomagen Inc. can provide raw data, basic analysis and/or customized analysis depending on the requirements of the research project.

What Is Whole Exome Sequencing?

using tweezers to pick part of genetic linkage

Most of eukaryotic species has exons, known as the exome, which is the protein-coding regions of the gene.

Whole exome sequencing is a targeted approach that makes it possible to identify variations in the protein-coding part of a selected gene since only about 2% of the whole genome is made up with exomes. This can be especially valuable because most known genetic variants actually occur in exons. With whole exome sequencing, DNA of known organism is mapped out using a reference genome, and specific genetic mutations and disease-causing variants are identified.

Psomagen Inc. uses only the most advanced whole exome sequencing research methods available, including next-generation sequencing platforms. This technology has completely revolutionized DNA sequencing, make it possible for researchers to process and decode exomes in as little time as possible, sometimes in just 24-48 hours.

Benefits of Whole Exome Sequencing

Whole exome sequencing can be extremely cost-effective, while other methods of DNA sequencing can be more time- and cost-consuming. Whole exome sequencing takes less time to complete, as it targets specific nucleotides found in the exome, and higher coverage can be obtained at a fraction of the cost compared to Whole Genome Sequencing.

Workflow

Psomagen Inc. offers optimized, wide-ranging enrichment methods, sequencing platforms, and sequencing coverage available. Researchers can improve processing, speed up workflow, and deliver comprehensive capture solutions for all needs.

Enrichment methods

  • Agilent SureSelect Exome Capture

Sequencing platforms

  • NovaSeq6000 (Illumina)
  • HiSeq2500/4000 (Illumina)

Sequencing Coverage

  • Minimum of 50X with no maximum

Data Analysis

  • Variant Calling & Analysis
  • Annotation
  • Comparative Analysis (Cancer, Family, Population, etc.)

Inquire About Our Exome and Targeted Genome Services