RNA Sequencing Services

About RNA Sequencing Services

Psomagen offers high-quality RNA sequencing services, also called transcriptome sequencing. Our state-of-the-art automated sequencing reduces the turnaround time for researchers at low prices, yet retains the highest levels of quality. Our RNA / transcriptome sequencing methods are reliable, fast, and cost-effective. We also provide bioinformatics services regarding differentially expressed genes, fusion genes, post-translation modification of specific mRNA transcripts, and more. We offer RNA / transcriptome sequencing services for researchers in the United States and around the globe.

What Is RNA/Transcriptome Sequencing?

What Is a Transcriptome?

A transcriptome is a collection of all the transcripts present in a given cell. An RNA sequence mirrors the sequence of the DNA from which it was transcribed, so by analyzing the entire collection of RNAs or transcriptomes in a cell, researchers can determine when and where each gene is turned on or off in the cells and tissues of an organism.

Why Is Transcriptome Sequencing Important?

RNA sequencing gives researchers more visibility into previously undetected changes that occur in certain states of diseases. Using RNA sequencing, researchers can learn more about a transcriptome’s new and existing qualities in just one assessment. They can also see how a transcriptome reacts to certain therapies, environmental situations, and other conditions. RNA sequencing even reveals alternative gene-spliced transcripts, gene fusion, mutations and variants, post-transcriptional modifications, and other gene expressions.

As DNA sequencing technology advances, RNA/transcriptome sequencing becomes more affordable and widely available. This has made RNA sequencing an excellent tool for RNA editing, RNA profiling, and allele-specific expression.

researcher doing lab work

Types of RNA/Transcriptome Sequencing

Science utilizes many different methods of RNA and transcriptome sequencing to identify existing and novel types of gene expression. At Psomagen Inc., we use only the most advanced and industry-standard methods, including the following:

  • mRNA Sequencing: This type of RNA sequencing is one of the most popular methods of analyzing disease states and biological processes in the study of transcriptomes. This method is extremely in-depth and allows researchers to find new genes that were previously imperceptible.
  • Small RNA Sequencing: This type of sequencing targets and isolates small RNA, including microRNA, to discover how noncoding RNA affects different types of gene expressions.
  • Whole RNA Sequencing: Whole RNA sequencing is a comprehensive method that aims to accurately measure the number of genes and transcripts in the transcriptome. It identifies existing and novel coding and noncoding RNA.
  • Targeted RNA Sequencing: This sequencing method focuses on two different types of targeted transcriptome assessment: targeted enrichment and targeted amplicon.
  • RNA Exome Capture Sequencing: This method of RNA sequencing identifies different types of RNA exomes by examining specific regions of RNA coding.
  • Single-Cell RNA Sequencing: This type of RNA sequencing studies individual cells, specifically the transcriptomes and genomes. It allows researchers to examine the small complexities of each region of a transcriptome.

Benefits of RNA/Transcriptome Sequencing

Because this form of sequencing does not require prior knowledge about the transcript, it can identify information that might be overlooked by other methods. Some of its benefits include:

  • The ability to capture both previously known and novel transcriptome features
  • Close, accurate studies of a variety of gene expression
  • Reliable results — often at a lower price point per sample

RNA sequencing is precise in measuring transcripts and identifying complexities. It can more accurately determine gene expressions than many other methods of arrays.

How Psomagen’s RNA/Transcriptome Sequencing Services Work

Psomagen Inc. RNA/transcriptome sequencing services include the following:

  • Transcript isoform discovery and validation
  • Coding and annotation with SNPs
  • Profiling and characterization junctions
  • Quantitative determination and identification of regulatory transcripts

We take great care of your samples from beginning to end, regardless of which of our services you use. Our expert lab staff ensures quality results by adhering to strict quality assurance processes. Learn a little more about our RNA sequencing workflow here:

Sample extraction v2

Sample preparation:

Before RNA can be sequenced, the sample must be fragmented for next-generation sequencing (NGS).

Library preparation

Library preparation:

The next step involves attaching adapters to the ends of the fragments, such as the primary sequencing site and the amplification element, to make sequencing possible.

Sequencing v2

Sequencing:

Using NGS, the library can be sequenced. The extent of the library sequencing depends on what the data will be used for.

Raw data output and bioinformatics analysis

Raw data output and bioinformatics analysis:

In the last step, researchers conduct an RNA sequencing analysis to identify genes that are expressed in a unique way based on environment, biological factors, and more and draw hypotheses from the evidence.

How to Order RNA Sequencing Services

If you’d like to partner with Psomagen on your RNA/transcriptome sequencing project, the process is simple.

Online Account

Create a Psomagen account online. It’s easy — and it’s free. You will use this account to view documentation and oversee your projects with us.

What to Expect When You Work With Psomagen

When you make us your next-generation sequencing service provider, you can expect the latest solutions from our modern, fully equipped lab, and you’ll have full support from our clinicians during every phase of your research, whether it’s your first or your hundredth DNA project.

For more than 20 years, Psomagen Inc. has provided a wide variety of genetic technology services to more than 100 countries. We’re here to support you throughout your research.

Inquire About Our Transcriptome and RNA Sequencing Services