RNA Sequencing Services
Want greater insight into the molecular mechanisms of gene expression and how it changes under different conditions?
A transcriptome is a collection of all the transcripts present in a given cell. An RNA sequence mirrors the sequence of the DNA from which it was transcribed, so by analyzing the entire collection of RNAs or transcriptomes in a cell, researchers can determine when and where each gene is turned on or off in the cells and tissues of an organism.
RNA sequencing gives researchers more visibility into previously undetected changes that occur in certain states of diseases. Using RNA sequencing, researchers can learn more about a transcriptome’s new and existing qualities in just one assessment. They can also see how a transcriptome reacts to certain therapies, environmental situations, and other conditions. RNA sequencing even reveals alternative gene-spliced transcripts, gene fusion, mutations and variants, post-transcriptional modifications, and other gene expressions.
As DNA sequencing technology advances, RNA/transcriptome sequencing becomes more affordable and widely available. This has made RNA sequencing an excellent tool for RNA editing, RNA profiling, and allele-specific expression.
Science utilizes many different methods of RNA and transcriptome sequencing to identify existing and novel types of gene expression. At Psomagen Inc., we use only the most advanced and industry-standard methods, including the following:
Before RNA can be sequenced, the sample must be fragmented for next-generation sequencing (NGS).
The next step involves attaching adapters to the ends of the fragments, such as the primary sequencing site and the amplification element, to make sequencing possible.
Using NGS, the library can be sequenced. The extent of the library sequencing depends on what the data will be used for.
Raw data output and bioinformatics analysis:
In the last step, researchers conduct an RNA sequencing analysis to identify genes that are expressed in a unique way based on environment, biological factors, and more and draw hypotheses from the evidence.
If you’d like to partner with Psomagen on your RNA/transcriptome sequencing project, the process is simple.
Request a no-obligation quote via email, contact form, or phone. Our team will get back to you promptly.
Create a Psomagen account online. It’s easy — and it’s free. You will use this account to view documentation and oversee your projects with us.
When you make us your next-generation sequencing service provider, you can expect the latest solutions from our modern, fully equipped lab, and you’ll have full support from our clinicians during every phase of your research, whether it’s your first or your hundredth DNA project.
For more than 20 years, Psomagen Inc. has provided a wide variety of genetic technology services to more than 100 countries. We’re here to support you throughout your research.