What Is Sanger Sequencing?
Sanger Sequencing—also known as capillary electrophoresis sequencing (CES)—is the oldest proven process of sequencing regions of DNA by using oligonucleotide primers and chain termination.
It is commonly selected to sequence polymerase chain reaction (PCR) products, plasmid DNA, and bacterial artificial chromosomes (BACs).
Why Is Studying Sanger Sequencing Important?
The original sequencing method preceding next-generation sequencing (NGS), Sanger sequencing is useful as a low-cost, high accuracy method for interrogating small DNA regions in a limited number of samples.
How Is Sanger Sequencing Used?
Sanger sequencing meets many research and clinical needs. With Psomagen, you can conduct any of the sequencing projects listed below, or contact a customer service specialist to design a custom project. Our experts can help you get the results you need, with the support to interpret your data.
What Can You Do?
Confirm Clones
Verify correct insertion and orientation into plasmid and bacterial artificial chromosome (BAC) backbones.
Use Capillary Electrophoresis
Get quicker, more accurate services via automated capillary electrophoresis sequencing (CES).
Access Sequencing Expertise
Enlist Psomagen’s expert help as needed before, during, and after service.
Get Simplified Ordering
Learn how Psomagen’s barcode-based ordering system reduces form fills.
Technologies Used for Sanger Sequencing
Plasmid and PCR Product Sequencing 
- Detect the presence or absence of a target sequence in PCR products
- Use PCR prior to Sanger sequencing to create multiple copies of the sequence, making it easier to generate tagged fragments
- Determine identity and order of sequence by using primers targeting the plasmid backbone or insert sequence
- Available for Psomagen's EZ-Seq pre-order service
Rolling Circle Amplification (Direct Colony Sequencing)
- Directly sequence bacterial or fungal colony samples
- Amplify very small amounts of double- or single-stranded DNA templates
- Eliminate the need for plasmid preparation
You Can Do More with Sanger!
Genomic DNA Extraction
SNP Validation
Validations (CLIA/Research Use)
PCR Amplification
Plasmid Purification
Enzymatic PCR Product Identification
Fragment Analysis
Primer Design & Synthesis
RNA Sequencing (cDNA Synthesis)
Variant Reports (CLIA/Research Use)
Bacterial and Fungal Identification
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Looking for a Sample Dropbox Near You?
We have over 100 dropbox locations along the east coast.
If those don't work for you, use our Sanger sequencing form.
Why Our Clients Choose Psomagen
Superior Data Quality
Exceeds manufacturer benchmarks
Fast Turnaround
Starting at one week for sequencing projects
US-Based Processing
Sequencing and customer support in Rockville, MD
Ph.D. Level Support
Expert assistance every step of the way, with real-time project updates through our online system
Automated Workflows
Increase scalability and improve reproducibility
CLIA-Certified, CAP-Accredited Labs
Clinical research options available (inquire for more information)
Complete Sequencing Solutions
From extraction to data analysis, with many sample types accepted
Quotes From Our Clients
I've been using their sequencing services for ten years - still going back! Shipment is fast, instructions for preparing sample plates are clear, handling by technicians and communication by Psomagen is well-done.
Director
Botanical Research Institute
I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.
Professor
University Research Laboratory
I have used Psomagen for sequencing my clones for a site-directed mutagenesis screen. I enjoy the speed of delivery and the fact that I get my results before I wake up the next day. Customer care is present to answer all questions and is quick to resolve issues. I believe this is value for the money.
Biomedical Scientist
Rutgers University
I've been sending Sanger-sequencing and whole-plasmid-sequencing samples to Psomagen for a decade. I love and appreciate their service. They have great technicians who gladly re-queue my samples if there's an error. The best part is the quick turn-around-time. On Monday they picked up two of my plasmids from a dropbox. Four hours later, I had results. I don't know how they do it, but I'm grateful. Thank you.
Scientist
University of Maryland
Sanger Sequencing in Action
Step One Sanger, Step Two NGS
While Sanger sequencing is tried and true, some projects require the massively parallel sequencing provided by our NGS offerings.
