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Omics Research // Transcriptomics

Small RNA Sequencing

Target and isolate fewer than 200 nucleotides of small, non-coding RNA known to regulate transcription.

Let's Talk About Small RNA Seq

What Is Small RNA Sequencing?

The effective process of analyzing only small non-coding RNA fragments in a highly parallel sequencing method

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Why Is Small RNA Sequencing Important?

The major component of small non-coding RNAs are microRNA (miRNA). miRNA has important roles in cellular development including cell proliferation, cell death and apoptosis, cell fate, and developmental timing.

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What Can You Do?
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Non-Coding RNA Sequencing

Using total RNA samples or Psomagen extraction services

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tRNA and YRNA blocking

Increase microRNA reads

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Small RNA Analysis

Including composition, as well as known and novel miRNA prediction

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Precision Medicine

Uncover microRNA biomarkers for oncology precision medicine

We Enrich Total RNA Samples for:

microRNA (miRNA)

Small interfering RNA (siRNA)

Piwi-interacting RNA (piRNA)

Small nuclear RNA (snRNA)

Small activating RNA (saRNA)

Small rDNA-derived RNA (srRNA)

tRNA-derived small RNA (tsRNA)

Transfer RNA-Derived Fragments (tRFs) are even smaller fragments derived from mature tRNAs or pre-tRNAs, usually 14-32 nt in length.

On-Going Promotions

We're always adding new deals — check in often for updated promotions!

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Total RNA Sequencing Best Practices

Sample Preparation & RNA Extraction

RNA extraction from cells, tissue, FFPE, blood, and swab samples. We isolate high-quality total RNA with our extraction experts.

Have a low amount of samples? Take a look at our low-input RNA seq to find the right option for you.

Library Preparation

NEXTFLEX Small RNA-Seq Kit v3

  • 1ug Total RNA
  • Library QC: >2 nM at 100~300 bp

The recommended small RNA sequencing coverage targets ~10-20 million reads in depth. Psomagen typically guarantees raw sequencing depth.

Sequencing Instrumentation & Read Length

Illumina NovaSeq S1 or S2 (entire flow cell)

  • 35 bp read length

Bioinformatic Analysis

Basic analysis:

  • Mapping
  • Known/novel small RNA
  • Mature miRNA abundance
  • Small RNA composition
More on bioinformatics

Data Delivery

All sequencing run data is delivered as demultiplexed .fastq files

If entire flowcell or lane is purchased, the raw data can be delivered as BCL files if requested at time of contract

Data Delivery

All sequencing run data is delivered as demultiplexed .fastq files

If entire flowcell or lane is purchased, the raw data can be delivered as BCL files if requested at time of contract

Why Our Clients Choose Psomagen

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Superior Data Quality

Exceeds manufacturer benchmarks

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Fast Turnaround

Starting at one week for sequencing projects

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US-Based Processing

Sequencing and customer support in Rockville, MD

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Ph.D. Level Support

Expert assistance every step of the way, with real-time project updates through our online system

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Automated Workflows

Increase scalability and improve reproducibility

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CLIA-Certified, CAP-Accredited Labs

Clinical research options available (inquire for more information)

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Complete Sequencing Solutions

From extraction to data analysis, with many sample types accepted

Quotes From Our Clients

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More Reviews on SelectScience®

I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.

Professor

University Research Laboratory

5-star
More Reviews on SelectScience®

I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.

Professor

University Research Laboratory

5-star
More Reviews on SelectScience®

I chose Psomagen because of the price and US-based labs. We've used Psomagen before and were very satisfied. The people were responsive and helpful and the work done was very good.

Professor

University Research Laboratory

We're Ready for Your Order!

Here's what to expect in your project's life cycle. If you have a question during sequencing, it's easy to get in touch!

01

Request a Consultation

Create an account through our client portal and get in touch with your local field team to start designing a project.

We’ll help drive your research forward with the right tools for your research needs.

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Place an Order

After finalizing the scope of work, the official quote will be issued for signing. Then, your sales representative will send specific shipping instructions.

Once all the samples and necessary documents are ready, we can start your project. Need more analysis? Reach out to our bioinformatics experts to dig deeper.

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QC and Data Delivery

All Psomagen sequencing projects come with a built-in QC step to ensure you get the information you need. An account manager will update you on your project's progress in a timely manner.

You can also see some project information and securely view all valid reports on the client portal site.

Customer Publications and Other Resources

More Customer Publications
A chromosome-level reference genome and pangenome for barn swallow population genomics
Whole genome sequencing identifies structural variants contributing to hematologic traits in the NHLBI TOPMed program
First complete mitochondrial genome of the Saharan striped polecat (Ictonyx libycus)

PSO Much More RNA Sequencing

mRNA Sequencing

Use the poly-A tail of mRNA transcripts to isolate the coding RNA sequences of actively transcribed genes. Obtain functional analysis of transcriptional regulators in disease and baseline biological processes.

Single-Cell RNA Sequencing

Analyze transcriptional changes on a cell-by-cell basis.

Sequence cellular diversity within a heterogeneous matrix for gene expression and precision medicine.

Total RNA Sequencing

For the most comprehensive data on the whole transcriptome. Encompass all RNA types and sizes recoverable via total RNA sequencing.

PSO Comprehensive!

What forms of RNA sequencing will get you the insights you need?

Ask Our Experts