What Is Total RNA Sequencing?
The next-generation sequencing process of sequencing mRNA with the inclusion of non-coding RNA (ncRNA).
Why is Total RNA Sequencing Important?
RNA transcription is the link between DNA and protein expression.
Studying the RNA present gives scientists insight into the biology of a cell and how changes — alternative splicing events, gene fusions, and allele-specific expression of rare and novel messages — may relate to disease.
What Can You Do?
Ribosomal RNA Depletion
Remove abundant rRNA molecules to focus on the coding and non-coding transcripts of interest
Strand-Specific Sequencing
Identify which strand of DNA the transcript was derived from
Experienced Bioinformatics Experts
Basic gene expression and SNV/InDel or advanced bioinformatic analysis services
Technologies Used for Total RNA Sequencing
rRNA Depletion
Total RNA sequencing uses a ribosomal RNA (rRNA) depletion step to remove the majority of rRNA before preparing libraries of your RNA. This negative selection approach enables the researcher to increase the amount of relevant sequencing reads and examine:
- messenger RNA (mRNA)
- long non-coding RNA (lncRNA)
- small non-coding RNA (sncRNA)
Stranded RNAseq
Psomagen uses strand-specific total RNA sequencing, or stranded RNAseq, to identify overlapping transcripts and genes.
Researchers can use the strand orientation information in their analyses to aid in annotation and novel transcript calls.
Low-Input Stranded RNAseq 
Don’t have a lot of RNA? Use our low-input stranded RNAseq service. This service still provides high quality data for both coding and non-coding RNA by enabling high sensitivity through changing the order in which the ribosomal RNA signal is targeted. The ribosomal RNA depletion step is skipped at the beginning of the workflow and alternatively, ribosomal cDNA depletion occurs after library assembly.
It is possible to use our low-input stranded RNAseq service with RNA input as low as 250pg.
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Why Our Clients Choose Psomagen
Superior Data Quality
Exceeds manufacturer benchmarks
Fast Turnaround
Starting at one week for sequencing projects
US-Based Processing
Sequencing and customer support in Rockville, MD
Ph.D. Level Support
Expert assistance every step of the way, with real-time project updates through our online system
Automated Workflows
Increase scalability and improve reproducibility
CLIA-Certified, CAP-Accredited Labs
Clinical research options available (inquire for more information)
Complete Sequencing Solutions
From extraction to data analysis, with many sample types accepted
